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Instrumentation
UTMB GCC 800 MHz NMR, 2006
Varian completed the installation of the 800 MHz H/C/N CryoProbe on the UTMB GCC 800 MHz NMR spectrometer in 2006. They achieved signal-to-noise (S/N) ratio of 5984:1, about three times the RT probe (S/N=2000:1) which meets specifications.
Operations
Dr. Vince Hilser, Director of the Sealy Center for Structural Biology, serves as contact. Dr. Tianzhi Wang serves as laboratory manager. For members of the GCC, the instrument is available on a fee-for-service basis ($8/hr).
Training
The lab manager offers personalized instruction in the operation of the instrument. Instrument competence is required for a user to qualify for unassisted use.
Rice GCC 800 MHz NMR, 2006
In June of 2006, the H/C/N cold probe was installed on the NMR. This probe substantially increased the sensitivity of the instrument (an s/n of ca. 6000:1 on the proton standard). In September of 2006, the cryogenic switch opened again for the 3rd time, but was rapidly returned to service. An agreement with Varian and Magnex on service for this continuing issue is largely complete.
Operations
Dr. Edward Nikonowicz, Vice Director of the GCC MR, serves as the contact at Rice for the 800. Dr. Sean Moran serves as laboratory manager. For members of the GCC, the instrument is available on a fee-for-service basis ($8/hr).
Training
The lab manager offers personalized instruction in the operation of the instrument. Instrument competence is required for a user to qualify for unassisted use.
NMR Microimaging Laboratory, Baylor College of Medicine
Background
The original Keck proposal provided $500,000 to BCM to establish a shared animal MRI Laboratory. In 2006 an application was submitted by Dr. Morrisett to the NIH Shared Instrumentation Program for a Magnetic Resonance Microimaging system. The instrument was funded in the amount of $329,900 that was used to purchase a 9.4T (89mm bore) superconducting magnet with a Bruker AVANCE console with capabilities for imaging animal and human tissues as well as live mice. For mouse imaging, the system is equipped for anesthesia administration and cardiac gating. In response, the institution renovated space in its Neurosensory vivarium (NB 531) to accommodate the instrument near the mice to be imaged on it.
Operations
Joel Morrisett, PhD serves as Director of the laboratory and Christof Karmonik, PhD as laboratory manager. For members of the GCC, the instrument is available on a collaborative basis. For other users, the instrument is operated on a fee-for-service basis in which assisted ($50/hr) and unassisted ($25/hr) use of the instrument is provided.
Training
The director offers a series of 4 MRI and MRS lectures through the Cardiovascular Sciences Graduate Core Course. The lab manager offers personalized instruction in the operation of the instrument. Instrument competence is required for a user to qualify for unassisted use. Although this facility is within the Baylor transgenics vivarium, arrangements can be made to image mice brought from the outside of the barrier for non-Baylor researchers.
The Rice NMR inventory in the Biochemistry department is:
Varian Inova 500 w/HCNP quad probe
Varian Inova 600 w/HCN cold probe (H and C double-enhanced)
Varian Inova 800 w/HCN cold probe (H only enhanced); GCC MR instrument
UTMB NMRs within the Sealy Center for Structural Biology include:
Varian Direct Drive 750 w/HCN triple probe
Varian Direct Drive 800 w/HCN cold probe (H only enhanced); GCC MR instrument
Varian Inova 600 w/HCN triple probe
University of Houston NMR laboratory includes a Bruker Avance TM 800 w/HCN cryoprobe. Contact Xiaolian Gao, Professor of Biology and Biochemistry.
The UT-Houston NMR laboratory includes a Bruker AvanceTM 600 MHz NMR spectrometer system with 54 mm bore UltrashieldTM magnet, and electronics console capable of monitoring 4 channels, and a TXI (Inverse Triple Resonance) Cryoprobe (5 mm sample diameter) for observation of 1H while decoupling 13C and 15N, including 2D lock with actively shielded Z-gradient coil. Signal to noise is 1H > 6000:1 (0.1% ethyl benzene).
NMR at MDACC MDACC has a Bruker 300 MHz DPX and a Bruker 500 MHz DRX instruments on the North Campus and a Bruker 600 MHz Avance at the El Rio Facility.
The 300-MHz instrument has the following features:
A 5 mm broad band observe probe (BBO) for monitoring most magnetic nuclei
A custom built "quad" probe for observing 1H, 13C, 19F, and 195Pt. Changing nuclei is accomplished under software control
The 500-MHz instrument has the following features:
X, Y, and Z triple-axis pulsed field gradient capability
Six probes
1. 2.5-mm broad band observe probe for microsamples
2. 5-mm broad band observe probe
3. 5-mm broad band inverse detected probe
4. 5-mm triple resonance probe for observing 1H, 13C, and 15N
5. 5-mm triple resonance probe for observing 1H, 13C, and 31P
6. 10-mm custom built "quad" probe for observing 1H, 13C, 19F, and 31P in biological samples such as cultured cells. Changing nuclei is accomplished under software control
A Case autosampler, which can process up to 24 samples, is available for use with either spectrometer.
The 600-MHz spectrometer has the following features:
Z-axis pulsed field gradient capability
Seven Probes
1. 5-mm broad band observe probe
2. 5-mm broad band inverse detected probe
3. 5-mm triple resonance probe for observing 1H, 13C, and 31P
4. 5 mm dual resonance probe tunable to 1H and 19F
5. 10-mm custom built "quad" probe for observing 1H, 13C, 19F, and 31P in biological samples such as cultured cells. Changing nuclei is accomplished under software control
6. High Resolution Magic Angle Spinning probe for solids tunable to 1H and 13C
7. High Resolution Magic Angle Spinning probe for solids tunable to 1H and 31P
For more information, contact:
Rice University
Sean Moran, Ph.D.
BioNMR Facility Manager
Phone: 713-348-4255
Email: seamus@rice.edu
UT Medical Branch at Galveston
Tianzhi Wang, Ph.D.
Research Scientist
Phone: 409-747-6821
Email: tiawang@utmb.edu
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